clec4f unconjugated  (R&D Systems)

Journal: Cell

Article Title: Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches

doi: 10.1016/j.cell.2021.12.018

Figure Lengend Snippet: Evolutionarily conserved signals regulate LAM and KC development, related to and (A) Confocal microscopy of healthy human liver showing expression of indicated markers. Scale bars, 200 μm. (B and C) Expression of conserved human-murine bile-duct LAM signature in human (B) and mouse (C) hepatic myeloid cells. (D) Proportion of indicated myeloid cell populations as a % of total myeloid cells in human liver biopsies profiled by scRNA-seq when divided based on presence of steatosis. (E) Mice were fed a western diet (WD) or standard diet (SD) for 36 weeks to induce NAFLD and Visium analysis was performed. Analysis is pooled from 1 liver slice from the SD condition and 3 liver slices from the WD condition. Shown are cluster and sample annotations. (F) Zonation of all cell types from Figure S5 J in murine NAFLD map (SD&WD). (G) Differential NicheNet highlighting prioritized conserved (human-mouse) ligand-receptor (LR) pairs between indicated macs and their niche cells. LR pairs are grouped according to the niche cell type with highest ligand expression. (H) Expression of ALK1 (ACVRL1), BMP9 ( GDF2 ), and BMP10 in human, mouse, and macaque livers where both KCs and stellate cells were profiled. (I) Livers were harvested from Clec4f -Crex Acvrl1 fl/fl mice or Acvrl1 fl/fl controls and KCs examined (top) and quantified (middle) using VSIG4 expression. Expression of indicated KC markers by mac populations in Clec4f -Crex Acvrl1 fl/fl or Acvrl1 fl/fl control mice (bottom). Data are pooled from 2 independent experiments with n = 14 per group. Student’s t test. ∗∗∗∗ p < 0.0001. (J) Expression of CD31 (ECs), DESMIN (stromal cells), F4/80 (Macs), and EPCAM (cholangiocytes) by confocal microscopy in Fcgr1 - Cre x Acvrl1 fl/fl mice and Acvrl1 +/+ controls. PV, portal vein; CV, central vein. Images are representative of 2 mice per group.

Article Snippet: Rat Monoclonal Clec4F-Unconjugated (370901) , R & D Systems , MAB2784; RRID: AB_2081338.

Techniques: Confocal Microscopy, Expressing, Western Blot

Journal: Cell

Article Title: Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches

doi: 10.1016/j.cell.2021.12.018

Figure Lengend Snippet: ALK1-BMP9/10 axis regulates KC development (A) Mouse BM monocytes were cultured in the presence of CSF1 and indicated concentrations of human ac-LDL, prior to analyzed for expression of indicated genes by qPCR. Data are pooled from 2 experiments. One-way ANOVA with Bonferroni post-test compared with 0 ng/mL. (B) NicheNet circos plot highlighting conserved ligand-receptor pairs and induced target genes between KCs and indicated niche cells in human and mouse. (C) Feature plots showing expression of ALK1 ( Acvrl1 ) in human myeloid cells (left) and GDF2/BMP10 in CD45 − cells (right). (D) Livers were harvested from Fcgr1 -Crex Acvrl1 fl/fl mice or Acvrl1 fl/fl or Acvrl1 +/+ controls and KCs examined (left) and quantified (right) using VSIG4 expression. (E) Expression of indicated KC markers by mac populations in Fcgr1 -Crex Acvrl1 fl/fl or Acvrl fl/fl control mice. Data are pooled from 3 independent experiments with n = 9 per group. Student’s t test. (F) Expression of indicated markers in livers of Fcgr1 -Crex Acvrl1 fl/fl or Acvrl1 fl/fl or Acvrl1 +/+ control mice by confocal microscopy. Scale bars, 50 μm. Images are representative of 2 mice per group. (G) Schematic of chimera experiment setup. (H) % chimerism normalized to levels in blood Ly6C hi monocytes in Clec4f-Dtr mice 7 or 13 days after DT administration following partial irradiation and receiving either Acvrl fl/fl or Fcgr1-CrexAcvrl fl/fl BM 4 weeks earlier. Data are pooled from 2 independent experiments with n = 10–12 mice per group. (I) Schematic of Fc trap experiment setup. (J) Representative FACS plots showing VSIG4 and CLEC2 expression by total macs. Numbers represent % of total mac population in the indicated gate (left) and % of VSIG4 + and VSIG4 − macs among total CD45 + cells in the different treatment conditions. One-way ANOVA with Bonferroni post-test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. See also Figure S8 .

Article Snippet: Rat Monoclonal Clec4F-Unconjugated (370901) , R & D Systems , MAB2784; RRID: AB_2081338.

Techniques: Cell Culture, Expressing, Confocal Microscopy, Irradiation

Journal: Cell

Article Title: Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches

doi: 10.1016/j.cell.2021.12.018

Figure Lengend Snippet:

Article Snippet: Rat Monoclonal Clec4F-Unconjugated (370901) , R & D Systems , MAB2784; RRID: AB_2081338.

Techniques: Purification, Recombinant, Staining, Expressing, Software, Microscopy